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AQuora® Fluorescein-NHS Ester (QBD-10885LF)

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AQuora® Fluorescein-NHS Ester is a xanthene-based, amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. It is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Description

“AQuora® Fluorescein-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein NHS Ester is a xanthene-based dye with photophysical properties comparable to FITC, carboxyfluorescein, and Alexa Fluor® 488 (absorption maximum = 495 nm, emission maximum = 524 nm).
This AQuora® Fluorescein NHS Ester dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.”

Specifications

Unit Size1mg
FormatSolid
StorageStore at -20°C. Protect from light. May ship at ambient temperatures.
Country of OriginUnited States of America
LabelAQuora® Fluorescein
Excitation / Emission Maximum495 nm / 524 nm
A280 Correction Factor0.190
Reactive GroupNHS Ester
Extinction Coefficient76,000
Spectrally Similar DyesFITC and Carboxyfluorescein
Molecular Weight1090

Applicable patents and legal notices are available at legal notices.

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