AQuora® Fluorescein-NHS Ester is a xanthene-based, amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. It is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.
“AQuora® Fluorescein-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein NHS Ester is a xanthene-based dye with photophysical properties comparable to FITC, carboxyfluorescein, and Alexa Fluor® 488 (absorption maximum = 495 nm, emission maximum = 524 nm).
This AQuora® Fluorescein NHS Ester dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.”
| Unit Size | 1mg |
|---|---|
| Format | Solid |
| Storage | Store at -20°C. Protect from light. May ship at ambient temperatures. |
| Country of Origin | United States of America |
| Label | AQuora® Fluorescein |
| Excitation / Emission Maximum | 495 nm / 524 nm |
| A280 Correction Factor | 0.190 |
| Reactive Group | NHS Ester |
| Extinction Coefficient | 76,000 |
| Spectrally Similar Dyes | FITC and Carboxyfluorescein |
| Molecular Weight | 1090 |
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