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AQuora® 550-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® 550-NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 550-NHS Ester is a cyanine-based dye with photophysical properties comparable to Alexa Fluor® 555, Cy3, DyLight® 550, and DyLight® 550 2x PEG.
This AQuora® 550-NHS Ester dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.
| Unit Size | 1mg |
|---|---|
| Format | Lyophilized |
| Storage | Store at -20°C. Protect from light. May ship at ambient temperatures. |
| Country of Origin | United States of America |
| Label | AQuora® 550 |
| Excitation / Emission Maximum | 554 nm / 570 nm |
| A280 Correction Factor | 0.080 |
| Reactive Group | NHS Ester |
| Extinction Coefficient | 150,000 |
| Spectrally Similar Dyes | Alexa Fluor® 555, Cy3, DyLight® 550, and DyLight® 550 2x PEG |
| Molecular Weight | 1530 |
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