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Goat Anti-Rabbit IgG Antibody (H+L), AMCA (CI-1000-1.5)

The product has been discontinued

Please consider the following as potential substitutes: DI-1088-1.5. Horse Anti-Rabbit IgG Antibody (H+L), DyLight™ 488. Or contact Technical Support @ [email protected] for assistance in finding alternative.

Description

AMCA Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 350 nm
  • Emission: 450 nm
  • Color: Blue

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Emission448-454 nm.
Maximum Excitation345-355 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml
Target SpeciesRabbit
ConjugateAMCA
Color of FluorescenceBlue
Host SpeciesGoat
FormatConcentrate

Citations

Technical Information

The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.

Applicable patents and legal notices are available at legal notices.