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Substrates play a crucial role in protein detection assays by enabling enzymatic reactions that generate colorimetric or chemiluminescent signals. These substrates are commonly used in immunohistochemistry (IHC), western blotting, and enzyme-linked immunosorbent assays (ELISA) to visualize target proteins with high sensitivity and specificity. Vector Laboratories provides high-quality substrate solutions.
- Alkaline Phosphatase (AP) Substrates – Designed for use with alkaline phosphatase (AP)-conjugated detection systems, these substrates produce distinct chromogenic or chemiluminescent signals for clear and reliable visualization in various immunoassays.
- DAB Enhancing Solution—Enhances the intensity and stability of 3,3′-Diaminobenzidine (DAB) substrate, a commonly used chromogen for horseradish peroxidase (HRP)-based detection, improving sensitivity and contrast in IHC and blotting applications.
- Horseradish Peroxidase (HRP) Substrates—Used in conjunction with HRP-conjugated antibodies, these substrates enable colorimetric or chemiluminescent detection, providing versatile options for high-contrast and high-sensitivity protein visualization.
By selecting the appropriate substrate, researchers can optimize signal clarity, enhance assay sensitivity, and achieve precise and reproducible protein detection results.
Explore Products Substrates
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ImmPACT® VIP Substrate Kit, Peroxidase (HRP)
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Vector® Black Substrate Kit, Alkaline Phosphatase (AP)
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ImmPACT® SG Substrate Kit, Peroxidase (HRP)
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Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
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AEC Substrate Kit, Peroxidase (HRP), (3-amino-9-ethylcarbazole)
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Vector NovaRED® Substrate Kit, Peroxidase (HRP)
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ImmPACT® AMEC Red Substrate Kit, Peroxidase (HRP)
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ImmPACT® AEC Substrate Kit, Peroxidase (HRP)
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Vector® SG Substrate Kit, Peroxidase (HRP)
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DuoLuX® Chemiluminescent and Fluorescent Substrate, Alkaline Phosphatase (AP)
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Vector® Blue Substrate Kit, Alkaline Phosphatase (AP)
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DAB Enhancing Solution
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DuoLuX® Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP)
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Vector® VIP Substrate Kit, Peroxidase (HRP)
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ImmPACT NovaRED® Substrate Kit, Peroxidase (HRP)
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DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3′-diaminobenzidine)
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ImmPACT® DAB Substrate Kit, Peroxidase (HRP)
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TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine)
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ImmPACT® Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
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BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium)
