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Vector NovaRED® Substrate Kit, Peroxidase (HRP) (SK-4800)

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Description

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 300 ml of working solution

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorRed
MicroscopyBrightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Multiple Labeling
MountingNon-Aqueous (Permanent)

Kit Contents:

  • 6 ml Vector NovaRED Reagent 1
  • 3.5 ml Vector NovaRED Reagent 2
  • 3.5 ml Vector NovaRED Reagent 3
  • 5 ml Vector NovaRED Reagent 4

Citations

Technical Information

Vector NovaRED and ImmPACT® NovaRED HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted. Both chromogens are useful as alternatives to DAB or as a second color for multiple antigen labeling. Vector NovaRED and ImmPACT NovaRED substrates also provide excellent color contrast in pigmented tissue such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield microscopy. Vector NovaRED and ImmPACT NovaRED substrates can be used for both manual and automated staining methods.

Vector NovaRED Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector NovaRED substrate is equivalent to Vector DAB Substrate, and 4 times greater than AEC. This product can also be used on blots. This kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 °C, this kit is stable for one year.

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Applicable patents and legal notices are available at legal notices.

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