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ImmPACT® AEC Substrate Kit, Peroxidase (HRP) (SK-4205)

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Description

ImmPACT AEC (3-amino-9-ethylcarbazole) HRP Substrate produces a red reaction product that can be used as a single label or as a second color for multiple antigen labeling. Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents. It generates a crisper reaction product that is 3-5 times more sensitive than conventional AEC substrates such as AEC Substrate Kit (SK-4200).

Features:

  • Optimized for IHC/ICC/ISH and blots
  • Working solution stable for up to 14 days at 4 °C
  • Supplied as 120 ml of diluent and stock solutions in dropper bottles

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorRed
MicroscopyBrightfield, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Multiple Labeling
MountingAqueous (Hardening), Aqueous (Non-Hardening)
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

Kit Contents:

  • 120 ml ImmPACT AEC Diluent
  • 3 ml ImmPACT AEC – Reagent 1
  • 3 ml ImmPACT AEC – Reagent 2
  • 3 ml ImmPACT AEC – Reagent 3

Product FAQs

We have offered conventional AEC peroxidase substrate, Cat. No. SK-4200, for many years. While this is our most economical kit format generating 300 ml of working solution, it is also the least sensitive of the three kits. Use of SK-4200 would be for the detection of abundantly expressed antigens and where assay costs are a main consideration. ImmPACT® AEC, Cat. No. SK-4205, was subsequently introduced and offers several advantages over the conventional SK-4200. In particular, SK-4205 is about 3-5 times greater in sensitivity over SK-4200, and the working solution is stable for two weeks when stored at 4°C. ImmPACT® AMEC Red, Cat. No. SK-4285, is our most sensitive AEC substrate that generates a crisper, brighter red reaction product, and is 5-10 times more sensitive when compared with conventional AEC substrates such as SK-4200. Like ImmPACT® AEC, the ImmPACT® AMEC Red working solution can be stored for two weeks in the fridge without loss of staining performance. We have also determined that where archiving of stained sections is important, ImmPACT® AMEC Red remains localized and undiminished in intensity for at least 2 years when coverslipped with VectaMount® AQ Mounting Medium.

Citations

Technical Information

AEC and ImmPACT AEC chromogens can be used for both manual and automated staining methods. Sections stained with AEC are stable for at least 4 years when mounted in VectaMount® AQ (H-5501).

This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT AEC reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4° C.

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Applicable patents and legal notices are available at legal notices.

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