Lectins & Glycobiology Reagents
Lectins are sugar-binding proteins found in plants, viruses, microorganisms, and animals. They bind glycoconjugates in much the same way as antibodies bind antigens. As a result, researchers use lectins to explore a myriad of biological structures and processes.
Lectins bind specific carbohydrate structures, so you can use them in glycobiology to:
- Separate oligosaccharides, even those with identical sugar compositions
- Discriminate between oligosaccharide structures
- Isolate a specific glycoconjugate, cell, or virus from a mixture
Some lectins can induce mitosis, so you can use them to investigate the process of lymphocyte blastogenesis and the biochemical and structural alterations associated with mitogenesis. These important applications include lymphokine production and viral coat protein (eg gp120) isolation.
Vector Laboratories — a leader in lectins and other glycobiology products
At Vector Laboratories, we employ crystallization, precipitation, and chromatographic procedures as well as affinity chromatography to purify our lectins. We monitor the purification process carefully and assess the final product through:
- Immunoprecipitation with antisera
- Agglutination titer
- Polyacrylamide gel electrophoresis
- Binding activity to specific affinity columns
We do this to ensure you have the best lectins available on the market.
Validation data for Vector's Lectins
A selection of Vector Laboratories’ lectin products is validated by the National Center for Functional Glycomics (NCFG) using the Consortium for Functional Glycomics (CFG) glycan arrays. The NCFG analyzes the glycan specificity of each lectin lot and makes the data available to the public on the NCFG website.
Explore Lectins & Glycobiology Reagents
Lectin screening kits provide a panel of lectins or lectin conjugates selected to provide a variety of sugar binding preferences. The smaller volume unit sizes in these kits are of the same high quality as individually offered lectin units.
All lectins manufactured by Vector Laboratories are extracted from a native plant source (i.e. seeds, bulbs, fruit etc.) and are not recombinant. Lectins are grouped based on their monsaccharide binding preferences.
Anti-lectin antibodies are offered in unconjugated or in biotinylated formats. In some applications, using biotinylated anti-lectins in conjunction with unlabeled lectins may provide greater sensitivity than using biotinylated lectins alone.
While the eluting solutions are primarily used in lectin agarose column and slurry preparations, inhibiting sugars, when combined with lectin solutions, have appeal as negative controls to prevent binding in IHC, IF, ELISA and blotting applications.
The Lectins Application and Resource Guide is a content rich educational booklet that provides insight into how lectins are applied in biological sciences. With a focus on plant derived lectins, the guide showcases the flexibility and utilization of lectins and lectin conjugates in established lab applications. Learn more about this guide.