The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
*Histochem J. (1998) 8:577
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Alkaline Phosphatase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Blue |
| Color of Fluorescence | Far Red |
| Microscopy | Brightfield, Fluorescence, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent) |
| Safety Title | WARNING: Reproductive Harm – www.P65Warnings.ca.gov |
Vector Blue is both chromogenic and fluorescent. Sections stained with the Vector Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount®, H-5000) must be used because the reaction product is soluble in xylene. Vector Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.
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