Cy5.5 Maleimide (also sold under as sulfo-Cyanine5.5 Maleimide) is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight 680™, and IRDye® 680 dye. Cy5.5 Maleimide is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents. Cy5.5 conjugates are pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||675/694 nm|
|Extinction coefficient||190,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Alexa Fluor® 680, DyLight® 680, IRDye® 680|
|Shipping Conditions||Ambient temperature|
How do I Request a Quote?
To request a quote for products: