Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||553/569 nm|
|Flow Cytometry Laser Line|
532, 555 or 568 nm
|Microscopy Laser Line|
532 or 555 nm
|Spectrally Similar Dyes||Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549|
|Molecular weight||806.96 (protonated)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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