MB 680R Azide

MB 680R Azide is a bright and very photostable probe routinely used for imaging of alkyne-containing biomolecules. MB 680R Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

MB 680R is a bright and photostable near IR dye that emits fluorescence at about 700 nm in the borderline spectral region between far-red and near-IR. MB 680R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable (Figure 1). The superior photostability and excellent brightness of MB 680R makes this dye an ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability.

MB 660R dye spectrally is almost identical to CF® 680R Dye and can be used a less expensive alternative to these dyes.

CF® Dye is a registered trademark of Biotium.