MB 594 NHS Ester is a very hydrophilic, water-soluble red fluorescent dye. Structurally related to Alexa Fluor® 594 dye, MB™ 594 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.
The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||605/635 nm|
|Extinction coefficient||110,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Alexa Fluor® 594, CF® 594, Texas Red|
|Shipping Conditions||Ambient temperature|
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