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Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated (BA-1000-1.5)

Purchase this product from 2BScientific

Description

The biotinylated goat anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques
  • Included in the VECTASTAIN® ABC kits

Specifications

Unit Size1.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin
Recommended UsageThe recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note: This product is at the same concentration as that provided in the VECTASTAIN® ABC kits.
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesGoat
FormatConcentrate

Citations

Technical Information

Biotinylated Goat Anti-Rabbit IgG (H+L) is supplied in liquid format. With some exceptions, the recommended dilution for most applications is 1:200.

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