The biotinylated goat anti-mouse IgM, mu chain specific secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. This mu chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins. It has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.
|Unit Size||0.5 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs|
|Recommended Storage||2-8 °C; Store frozen for long term storage|
|Solution||10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin.|
|Recommended Usage||The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.|
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies.
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