Format Concentrate | |
Formulation 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. | |
Unit Size 1.5 mg | |
Storage Instructions 2-8 °C; Store frozen for long term storage | |
Usage Summary The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. This product is at the same concentration as that provided in the VECTASTAIN® ABC kits. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Target Species Human_Target Species | |
Concentration 1.5 mg active conjugate/ml | |
Conjugate Biotinylated | |
Reactive Species Goat | |
Source Species Human_Source Species | |
Target BLANK | |
Host Species Goat |
Goat Anti-Human IgG Antibody (H+L), Biotinylated
The biotinylated goat anti-human IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features::
• Purified, ultrapure, high affinity antibody
• Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
• Recognizes both heavy and light chains (H+L)
• Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
• Included in the VECTASTAIN® ABC kits
• Supplied in solution
$208.00
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Technical Information Vector Laboratories affinity-purified antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Biotinylated Goat Anti-Human (H+L) IgG is supplied in solution form. With some exceptions the recommended dilution for most applications is 1:200. |
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