AZDye 647 Amine (FP-1123)

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Description

647Nm Laser

AZDye™ 647 is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AZDye 647 is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer, a reactive dye for labeling proteins via a carboxylic acid moiety, and amine donor for enzymatic transamination labeling.

AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima649/671 nm
Extinction coefficient270,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesCy5, DyLight® 649,
Molecular weight915.12 (protonated)
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Af647

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Applicable patents and legal notices are available at legal notices.

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