AZDye™ 647 is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
AZDye 647 is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer, a reactive dye for labeling proteins via a carboxylic acid moiety, and amine donor for enzymatic transamination labeling.
AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||649/671 nm|
|Extinction coefficient||270,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Cy5, DyLight® 649,|
|Molecular weight||915.12 (protonated)|
|Shipping Conditions||Ambient temperature|
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