AZDye 594 Alkyne

AZDye™ 594 Alkyne is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.

For application where the presence of copper is not acceptable, please consider our AZDye™ 594 DBCO probe for copper-less detection of azide-modified molecules.

Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.