AZDye™ 594 Alkyne is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.
For application where the presence of copper is not acceptable, please consider our AZDye™ 594 DBCO probe for copper-less detection of azide-modified molecules.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||590/617 nm|
|Flow Cytometry Laser Line|
561 nm or 594 nm
|Microscopy Laser Line|
|Spectrally Similar Dyes||Alexa Fluor® 594, CF® 594, DyLight® 594|
|Molecular weight||758.18 (protonated)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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