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AZDye™ 546 Alkyne is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AZDye™ 546 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines.
For application where the presence of copper is not acceptable, please consider our AZDye™ 546 DBCO or MB 543 DBCO probes for copper-less detection of azide-modified molecules.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 543/563 nm |
| Extinction Coefficient | 110,000 |
| Flow Cytometry Laser Line | 532 nm |
| Microscopy Laser Line | 543 nm or 546 nm |
| Spectrally Similar Dyes | Alexa Fluor® 546, Atto™ 546, CF® 546 |
| Molecular weight | 856.98 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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