AZDye™ 488 Alkyne is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AZDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less azide detection (AZDye™ 488 DBCO) is also available for application where the presence of copper is not acceptable.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488 Alkyne. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488 and CF® 488 Dye.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||494/517 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
|Spectrally Similar Dyes||FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488|
|Molecular weight||571.53 (protonated)|
|Solubility||Water, DMSO, DMF|
|Appearance||Orange to light red solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
How do I Request a Quote?
To request a quote for products: