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AZDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AZDye™ 488 is green-emitting dye with excitation ideally suited for the 488 nm line. Its fluorescence is pH independent from pH 4 to pH 10.
AZDye™ 488 Acid often used for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), and STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for the non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, e.g. HATU.
| Unit Size | 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Primary amines |
| Abs/Em Maxima | 494/517 nm |
| Extinction coefficient | 73,000 cm-1M-1 |
| Solubility | Water, DMSO, DMF |
| Spectrally similar dyes | Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488 |
| Molecular weight | 534.47 (protonated) |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
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