AZDye™ 405 Acid is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.
The carboxylic acid of AZDye™ 405 is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), and STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for the non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, e.g. HATU. AZDye™ 405 Acid is also often used for control experiments, and for calibration.
|Unit Size||5 mg, 25 mg, 100 mg|
|Reactivity||Primary amines (requires activation)|
|Abs/Em Maxima||402/424 nm|
|Extinction coefficient||35,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Alexa Fluor® 405, DyLight® 405, Cascade Blue, CF® 405|
|Molecular weight||629.62 (protonated)|
|Shipping Conditions||Ambient temperature|
How do I Request a Quote?
To request a quote for products: