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This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.
Unit Size | 50 ml |
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Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
Concentration | Ready-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
Recommended Storage | 2-8 °C |
Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl. |
Target Species | Universal (Mouse/Rabbit) |
Conjugate | Biotinylated |
Host Species | Horse |
Format | Ready-to-Use |
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