4-Azido-L-phenylalanine is an unnatural derivative of L-phenylalanine that can be incorporated by recombinant methods into proteins allowing site-specific modification, e.g. by ‘click chemistry’, Staudinger reaction, or by UV-irradiation at 254 nm. The azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
Unit Size | 1 g, 5 g, 25 g |
---|---|
Molecular weight | 206.20 |
Chemical composition | C9H10N4O2 |
CAS | 33173-53-4 |
Solubility | Water, DMSO, DMF |
Purity | >95% (H NMR) |
Appearance | Off-white to slightly grey crystalline |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
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