Observation is one of the fundamental steps in the scientific method. However, for centuries the scientific study of tissues was limited to observations of dissections with the unaided eye. It was not until the mid-19th century with the introduction of dyes, such as hematoxylin, that the subcellular structure of tissues became visible and the science of histochemistry emerged (1).
Avidin-Biotin Complex, or ABC-based detection, is one of the most widely used methods for staining. This method exploits the high affinity exhibited between the protein avidin and the vitamin biotin forming large macromolecular complexes containing multiple enzyme molecules—either horse radish peroxidase (HRP) or alkaline phosphatase (AP).
This blog post covers lectin diversity and where lectins come from.
In this blog post, we will explore IHC and IF techniques and how they have been used for identifying cancer biomarkers.
In this blog post, we will explore IHC and IF techniques and how they have been used for identifying cancer biomarkers.
With a wide variety to choose from, there are important considerations to make when selecting your own primary antibody. Keep reading to learn how to choose a primary antibody for your workflow.
This blog will discuss the considerations in choosing a secondary antibody for IHC.
This blog will discuss some miscellaneous factors to focus on when an IHC or IF protocol performs unexpectedly.
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