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5′ EndTag™ Nucleic Acid Labeling Kit (MB-9001)
Texas Red Maleimide can be used to label free thiol groups with this high quantum yield rhodamine derivative. This tag can either be directly visualized or detected using anti-rhodamine antibodies. Texas Red Maleimide is designed for fluorescent labeling of proteins, nucleic acids or other molecules containing one or more thiol groups.
| Unit Size | 3.6 mg |
|---|---|
| Target for Labeling | DNA, Oligonucleotides, PNA, Protein, RNA |
| Recommended Storage | – 20°C to – 80 °C. |
| Molecular Weight | 728 |
| Chemical Formula | C37H36N4O8S2 |
| Maximum Emission | 614nm |
| Maximum Excitation | 592nm |
| Required Reactive Group | Thiol Group |
| Tag/Group Incorporated | Texas Red |
| Safety Title | WARNING: NA – www.P65Warnings.ca.gov |
Molecules to be labeled with Texas Red Maleimide require free thiol groups. In the case of proteins, Texas Red Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).
For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.
This fluorescent label is a high quantum yield rhodamine derivative that can be directly visualized. The label can also be detected, and the signal amplified, using our affinity-purified antibody conjugates to rhodamine. (Excitation maximum at 595 nm; emission maximum at 615 nm).
Applicable patents and legal notices are available at legal notices.
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