| Unit Size | 50 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | Ready-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
| Recommended Storage | 2-8 °C |
| Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl. |
| Target Species | Mouse |
| Conjugate | Biotinylated |
| Host Species | Goat |
| Format | Ready-to-Use |
Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.
Biotinylated Goat Anti-Mouse IgG (H+L) is supplied in solution. With some exceptions the recommended dilution for most applications is 1:200.
(H+L) indicates the antibody recognizes both heavy and light chains.
Applicable patents and legal notices are available at legal notices.
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