OG 488 Alkyne (CCT-1397)

Description

OG 488 Alkyne, (also known as Oregon Green® 488 Alkyne or 2′,7′-Difluorocarboxyfluorescein Alkyne, 6-isomer) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. OG 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

The fluorinated carboxyfluoresceins have higher photostability and ionize at a lower pH (pKa 4.8) than fluorescein (pKa 6.5), which makes them superior fluorescent dyes for use as reporter molecules in biological systems. The absorption and emission of OG 488 dye are virtually identical to widely used fluorescein dyes.

The combination of higher photostability, lower pKa, and excitation ideally suited to the 488 nm laser line makes OG 488 dye an ideal replacement for the widely used fluorescein dyes.

Oregon Green® is a registered trademark of Thermo Fisher Scientific.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima496/524 nm
Extinction Coefficient
84,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar DyesFluorescein, Alexa Fluor® 488, Atto™ 488, CF® 488A dyes, DyLight® 488
Molecular weight449.37
CASN/A
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceOrange to red solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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