NHS-biotin

NHS-biotin, product number QBD-10205, is a classic, non-dPEG® biotinylation reagent. Biotin (5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, CAS number 58-85-5) is a water-soluble vitamin, though it is not especially soluble in water and is used in a wide range of biotechnology and clinical applications. NHS-biotin (2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, CAS number 35013-72-0) is the N-hydroxysuccinimide ester of biotin. The NHS ester is reactive with free primary amines. It is useful for labeling proteins, peptides, surfaces (such as silica, glass, or magnetic particles) coated with primary amines, and the like. It can also be used with amine-terminated dPEG® products to create novel biotinylated dPEG® products.

NHS-biotin, QBD-10205, is insoluble in water, but the NHS ester hydrolyzes if the product gets wet. The optimal storage temperature of NHS-biotin is -20°C. When removed from storage, NHS-biotin should be allowed to equilibrate fully to ambient temperature before opening the bottle. The product should always be handled to minimize exposure to moisture. NHS-biotin dissolves in N,N’-dimethylacetamide (DMAC) or dimethylsulfoxide (DMSO). DMAC and DMSO must be dried over 3Å molecular sieves for at least 24 hours before use. Solutions of NHS-biotin should be used immediately. Storing NHS-biotin in solution is not recommended.