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PC Biotin-NHS Ester as a unique amine reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin beads. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).
| Unit Size | 4 x 1 mg, 10 mg, 25 mg, 100 mg |
|---|---|
| Molecular weight | 840.89 |
| Chemical composition | C36H52N6O15S |
| CAS | N/A |
| Solubility | DMSO, DMF, DCM, THF, Chloroform |
| Appearance | Slightly yellow amorphos solid to oil |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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