Molecular Weight 358 g/mol | |
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence | |
Formulation 10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide. | |
Unit Size 5 mg | |
Storage Instructions 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent. | |
Usage Summary Fixable with formaldehyde or glutaraldehyde | |
Neuronal Tracer - Direction of Transport Anterograde/Retrograde |
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This product has been discontinued.
Please consider the following as potential substitutes: SP-1120-20. NEUROBIOTIN Tracer. Or contact Technical Support @ [email protected] for assistance in finding alternative.
NEUROBIOTIN®-PLUS Tracer
NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling
Features:
• Fixable primary amine
• Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
• Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
• Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods
Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:
• Better solubility
• More efficiently iontophoresed
• Remains in cell longer
• Non-toxic
• Can be fixed with formalin or glutaraldehyde
$99.00
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Technical Information Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN-Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract. NEUROBIOTIN-Plus shares the same key advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:
NEUROBIOTIN-Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods. *Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138. |
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