NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.
|Unit Size||2 mg|
|Maximum Emission||517 nm|
|Recommended Storage||2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.|
|Recommended Usage||NEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO|
|Molecular Weight||747 g/mol|
|Maximum Excitation||493 nm|
|Neuronal Tracer - Direction of Transport||Anterograde/Retrograde|
|Detection Method||Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence|
NEUROBIOTIN 488 Tracer is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. NEUROBIOTIN 488 Tracer is readily fixed with standard aldehyde based fixatives. This tracer can be viewed directly under fluorescence microscopy.
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