Molecular Weight 114 | |
Color of Fluorescence Far Red | |
Extinction Coefficient 1.4 | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2. | |
Inhibiting or Eluting Sugar Galactose | |
Maximum Emission 670 nm | |
Maximum Excitation 655 nm | |
Unit Size 0.5 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity Terminal αGal | |
Usage Summary The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC. | |
Applications Immunofluorescence, Glycobiology | |
Concentration 1 mg active conjugate/ml | |
Conjugate DyLight 649 |
Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight 649
GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.
DyLight™ 649 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight™ 649 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.
• Excitation maximum: 646 nm
• emission maximum: 674 nm
• Color: Red
$202.00
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Technical Information GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. Inhibiting Sugar: 500 mM galactose or 100 mM raffinose |
Product FAQs
Can the biotinylated Lycopersicon esculentum (tomato) lectin be used for in vivo perfusion studies to trace blood vessels in mice?
The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112
