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Goat Anti-Human IgG Antibody (H+L), Fluorescein (FI-3000-1.5)

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Description

Fluorescein Goat Anti-Human IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with fluorescein to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 495 nm 
  • Emission: 515 nm 
  • Color: Green

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission510-520 nm
Maximum Excitation490-500 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If this fluorescein-labeled antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesHuman
ConjugateFluorescein
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

Citations

Technical Information

The goat anti-human Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

Fluorescein Goat Anti-Human IgG (H+L) Antibody can be used for flow cytometry or for tissue staining. 

Applicable patents and legal notices are available at legal notices.

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