Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.
Fluorescein labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
| Unit Size | 25 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. |
| Recommended Storage | 2-8 °C |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2. |
| Concentration | 5 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | Mannose, Glucose |
Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
Applicable patents and legal notices are available at legal notices.
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