Color of Fluorescence Red | |
Format Concentrate | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. | |
Maximum Emission 617 nm | |
Maximum Excitation 592 nm | |
Unit Size 1 mg | |
Storage Instructions 2-8 °C | |
Usage Summary Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. | |
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation | |
Concentration 1.0 mg/ml active conjugate | |
Conjugate DyLight 594 |
Streptavidin, DyLight™ 594
Features:
• Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
• Highly purified and possess very low non-specific binding properties
• Extremely high affinity for biotin
• Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
• Excitation at 592 nm
• Emission at 617 nm
• Color: Red
$202.00
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Technical Information Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. DyLight dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
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