Color of Fluorescence Green | |
Format Concentrate | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. | |
Maximum Emission 518 nm | |
Maximum Excitation 493 nm | |
Unit Size 1 mg | |
Storage Instructions 2-8 °C | |
Usage Summary Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. | |
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation | |
Concentration 1.0 mg/ml active conjugate | |
Conjugate DyLight 488 |
Streptavidin, DyLight™ 488
DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
Features:
• Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
• Excitation maximum: 493 nm
• Emission maximum: 518 nm
• Color: Green
$201.00
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Technical Information Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
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