Difluorocarboxyfluorescein NHS Ester, 6-isomer (FP-1223)

[text-backorder]

Description

488Nm Laser

Difluorocarboxyfluorescein NHS Ester, 6-isomer (also known as Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 6-isomer) is an amine-reactive fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Although the mixed isomers of Difluorocarboxyfluorescein NHS Ester, is a preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes.

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima496/524 nm
Extinction coefficient73,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein
Molecular weight509.38
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Mb488

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Applicable patents and legal notices are available at legal notices.

Related Products

How do I Request a Quote?

To request a quote for products:

  1.  Sign in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote