The Cy7 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
Cy7 Azide is a bright and photostable near-IR probe that is spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. The fluorescence of Cy7 Azide is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||753/775 nm|
|Spectrally Similar Dyes||Alexa Fluor® 750, RDye® 750, CF® 750 Dye, DyLight® 750|
|Solubility||Water, DMSO, DMF|
|Appearance||Dark green solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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