Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Unit Size | 1 mg, 5 mg |
---|---|
Abs/Em Maxima | 649/671 nm |
Extinction Coefficient | 250,000 |
Flow Cytometry Laser Line | 633 or 635 nm |
Microscopy Laser Line | 633 or 635 nm |
Spectrally Similar Dyes | Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 |
Molecular weight | 1136.39 |
CAS | N/A |
Solubility | Water, DMSO, DMF, MeOH |
Purity | >95% (HPLC) |
Appearance | Blue solid |
Storage Conditions | -20°C. Desiccate |
Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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