Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
| Unit Size | 1 mg, 5 mg |
|---|---|
| Abs/Em Maxima | 649/671 nm |
| Extinction Coefficient | 250,000 |
Flow Cytometry Laser Line | 633 or 635 nm |
| Microscopy Laser Line | 633 or 635 nm |
| Spectrally Similar Dyes | Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 |
| Molecular weight | 1136.39 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF, MeOH |
| Purity | >95% (HPLC) |
| Appearance | Blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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