Streptavidin, CY3

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

• Excitation: 552 nm
• Emission: 565 nm
• Color: Red

$213.00

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SKU: SA-1300-1
Color of Fluorescence
Red
Format
Concentrate
Formulation
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission
570 nm
Maximum Excitation
550 nm
Unit Size
1 mg
Storage Instructions
2-8 °C
Usage Summary
Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Concentration
1.0 mg/ml active conjugate
Conjugate
Cy3