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Cy3 Amine (FP-1303)

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This product was previously sold as 1415 from Click Chemistry Tools.

Description

Cy3 Amine is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.

Cy3 Amine (also sold under Sulfo-Cyanine3 Amine) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityAldehyde, carboxylic acid, ketone
Abs/Em Maxima555/572 nm
Extinction coefficient150,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesCy3, DyLight® 555, Alexa Fluor® 555
Molecular weight672.86
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy3 Cy3

Protein Labeling Calculator

[protein-labeling-calculator]

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