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Cy5 DBCO (CCT-A130)

[text-backorder]

This product was previously sold as 1327 from Fluoroprobes.

Description

Cy5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima649/671 nm
Extinction Coefficient
250,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar DyesAlexa Fluor® 647, CF™ 647 Dye, DyLight™649
Molecular weight1009.22
CAS1564286-24-3
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy5

Selected References

  1. Bazrafshan, A. et al. (2021). DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second. ACS Publications, Online ahead of print. [PubMed]
  2. Wiener, J., et al. (2020). Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics. Sci Rep.10 (1), 1457. [PubMed]
  3. Yong, K.W., et al. (2020). Engineering the Orientation, Density, and Flexibility of Single-Domain Antibodies on Nanoparticles To Improve Cell Targeting. ACS Appl. Mater. Interfaces12(5), 5593-600. [PubMed]
  4. Song, S., et al. (2020). In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjugate Chem.31(5), 1562-74. [PubMed]
  5. Valentini, T.V., et al. (2020). Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota. Nat Commun.11, 2287-74. [PMC]
  6. Zhang, C., et al. (2016). In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy. Proc Natl Acad Sci U S A.113 (19), 5173-8. [PubMed]
  7. Kim, S. H., et al. (2014). Cell labeling and tracking method without distorted signals by phagocytosis of macrophages . Theranostics4 (4), 420-31. [PubMed]

Applicable patents and legal notices are available at legal notices.

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