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Cy3 Azide (CCT-AZ119)

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This product was previously sold as 1304 from Fluoroprobes.

Description

Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima553/569 nm
Extinction Coefficient
150,000
Flow Cytometry Laser Line
532, 555 or 568 nm
Microscopy Laser Line
532 or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Molecular weight806.96 (protonated)
CAS1782950-79-1
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy3 Cy3

Selected References

  1. Leach, R. W., et al. (2021). Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Nat Chem Biol.17 (11), 1178-1187. [PubMed]
  2. Wiener, J., et al. (2020). Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics. Sci Rep.10 (1), 1457. [PubMed]

Applicable patents and legal notices are available at legal notices.

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