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Cy3 DBCO (CCT-A140)

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This product was previously sold as 1307 from Fluoroprobes.

Cy3 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

Description

Cy3 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. Cy3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy3 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.

Cy®3 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

This is sulfonated dye is also known as sulfo-Cyanine3.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima553/569 nm
Extinction Coefficient
150,000
Flow Cytometry Laser Line
532, 555 or 568 nm
Microscopy Laser Line
532 or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Molecular weight983.18
CAS1782950-79-1
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy3

Selected References

  1. Morana, O., et al. (2022). Identification of a New Cholesterol-Binding Site within the IFN-γ Receptor that is Required for Signal Transduction. Adv Sci (Weinh)e2105170, Online ahead of print. [PubMed]
  2. Daughtry, J. L., et al. (2020). Clickable Galactose Analogues for Imaging Glycans in Developing Zebrafish. ACS Chem Biol.15 (2), 318-324. [PubMed]
  3. Sato, S., et al. (2020). Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction. Bioconjugate Chem.31(5), 1463-73. [PubMed]
  4. Allo, B., et al. (2018). Clickable and High-Sensitivity Metal-Containing Tags for Mass Cytometry. Bioconjugate Chem. 29(6), 2028-38. [PubMed]

Applicable patents and legal notices are available at legal notices.

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