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The NIR-fluorescent Cy5.5 Alkynee can be reacted with azides (Ne) via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures.
Cy5.5 Alkyne is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. The Cy5.5 Alkyne is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy5.5 is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Abs/Em Maxima | 678/694 nm |
| Extinction Coefficient | 190,000 |
| Spectrally Similar Dyes | Alexa Fluor® 680, IRDye® 680RD, DyLight® 680 |
| Molecular weight | 940.09 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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