Cy3 Acid (FP-1300)

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Description

555Nm Laser

Cy3 Acid (also sold under Sulfo-Cyanine3 Acid) is a bright, water-soluble, and pH insensitive> from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

The carboxylic acid of Cy3 dye is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. Cy3 Acid is also often used for control experiments, and for calibration.

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityNon-activated
Abs/Em Maxima555/572 nm
Extinction coefficient150,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesCy3, DyLight® 555, Alexa Fluor® 555
Molecular weight616.74
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy3

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

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