CalFluor 580 Azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. This azide probe is not fluorescent until it is reacted with alkynes. CalFluor 580 Azide is a water-soluble probe, and its excitation band is a good match for the 568 nm or 591 nm laser lines.
A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.
To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.
|Unit Size||1 mg, 5 mg|
|Abs/Em Maxima, triazole after click reaction||591/609 nm|
|Spectrally similar dyes|
Alexa Fluor® 594, CF® 594, DyLight® 594, Texas Red
|Appearance||Dark red amorphous solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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