Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.
TAMRA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||553/575 nm|
|Flow Cytometry Laser Line|
532 nm or 555 nm
|Microscopy Laser Line|
532 nm or 555 nm
|Spectrally Similar Dyes||Alexa Fluor® 555, CF® 555, DyLight® 549|
|Solubility||Water, DMSO, DMF|
|Appearance||Dark red amorphous solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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