5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3).
Although the mixed isomers of 5(6)-TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.
|Unit Size||5 mg, 25 mg, 100 mg, 1000 mg|
|Abs/Em Maxima||553/575 nm|
|Extinction coefficient||91,000 cm-1M-1|
|Solubility||DMSO, DMF, MeOH|
|Spectrally similar dyes||Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543|
|Molecular weight||627.24 (TFA salt)|
|Shipping Conditions||Ambient temperature|
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