AZDye™ 594 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
5-TAMRA (also known as TAMRA, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||553/575 nm|
|Spectrally Similar Dyes||Alexa Fluor® 546, Atto™ 543, CF® 543 Dye|
|Appearance||Dark red amorphous solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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